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	Provedor de dados Genet. Mol. Biol. (2) ArchiMer (1) Ciência Rural (1) Electron. J. Biotechnol. (1) Autor Araújo,Ronyere Olegário de (1) Ballenghien, M. (1) Barbotte, L. (1) Barkley,Noelle A (1) Caetano,Alexandre Rodrigues (1) Dongzhi,Wei (1) Donnadieu, C. (1) Flahauw, Emilie (1) Gayral, P. (1) Genestout, L. (1) Haffray, P. (1) Harrang, Estelle (1) Heurtebise, Serge (1) Klopp, C. (1) Lapegue, Sylvie (1) Luan,Xiaohui (1) Mahla, R. (1) Pittman,Roy N (1) Sayed-Tabatabaei,Badraldin Ebrahim (1) Shahinnia,Fahimeh (1) Mais... Palavra-chave SNP genotyping (5) GoldenGate technology (2) Association studies (1) Barley (Hordeum vulgare L.) (1) Crassostrea gigas (1) Custom SNP panels (1) Fatty acid composition (1) Gas chromatography (1) Genome sequencing (1) Haplotypic blocks (1) Mismatched primer (1) Ostrea edulis (1) Oysters (1) PCR-RFLP (1) Peanut (Arachis hypogaea L.) (1) RER site (1) Real-time PCR (1) Restriction enzyme (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (1) Text (1) Ano 2016 (1) 2014 (1) 2011 (1) 2009 (1) 2006 (1) País Brazil (3) Chile (1) France (1) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing Genet. Mol. Biol. Xiao,Junhua; Xin,Xiujuan; Luan,Xiaohui; Dongzhi,Wei; Shengli,Yang. We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases. Tipo: Info:eu-repo/semantics/article Palavras-chave: SNP genotyping; PCR-RFLP; RER site. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300028 A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.) Electron. J. Biotechnol. Barkley,Noelle A; Wang,Ming Li; Pittman,Roy N. The high oleic (C18:1) phenotype in peanuts has been previously demonstrated to result from a homozygous recessive genotype (ol1ol1ol2ol2) in two homeologous fatty acid desaturase genes (FAD2A and FAD2B) with two key SNPs. These mutant SNPs, specifically G448A in FAD2A and 442insA in FAD2B, significantly limit the normal function of the desaturase enzyme activity which converts oleic acid into linoleic acid by the addition of a second double bond in the hydrocarbon chain. Previously, a genotyping assay was developed to detect wild type and mutant alleles in FAD2B. A real-time PCR assay has now been developed to detect wild type and mutant alleles (G448A) in FAD2A using either seed or leaf tissue. This assay was demonstrated to be applicable for the... Tipo: Journal article Palavras-chave: Fatty acid composition; Gas chromatography; Peanut (Arachis hypogaea L.); Real-time PCR; SNP genotyping. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100009 Conversion of barley SNPs into PCR-based markers using dCAPS method Genet. Mol. Biol. Shahinnia,Fahimeh; Sayed-Tabatabaei,Badraldin Ebrahim. Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker. Tipo: Info:eu-repo/semantics/article Palavras-chave: Barley (Hordeum vulgare L.); Genome sequencing; Mismatched primer; Restriction enzyme; SNP genotyping. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020 Development of SNP genotyping arrays in two shellfish species ArchiMer Lapegue, Sylvie; Harrang, Estelle; Heurtebise, Serge; Flahauw, Emilie; Donnadieu, C.; Gayral, P.; Ballenghien, M.; Genestout, L.; Barbotte, L.; Mahla, R.; Haffray, P.; Klopp, C.. Use of SNPs has been favored due to their abundance in plant and animal genomes, accompanied by the falling cost and rising throughput capacity for detection and genotyping. Here, we present in vitro (obtained from targeted sequencing) and in silico discovery of SNPs, and the design of medium-throughput genotyping arrays for two oyster species, the Pacific oyster, Crassostrea gigas, and European flat oyster, Ostrea edulis. Two sets of 384 SNP markers were designed for two Illumina GoldenGate arrays and genotyped on more than 1000 samples for each species. In each case, oyster samples were obtained from wild and selected populations and from three-generation families segregating for traits of interest in aquaculture. The rate of successfully genotyped... Tipo: Text Palavras-chave: Crassostrea gigas; GoldenGate technology; Ostrea edulis; Oysters; SNP genotyping. Ano: 2014 URL: http://archimer.ifremer.fr/doc/00172/28369/26677.pdf Low density genomic data for animal breeding: critical analysis and perspectives of the GoldenGate Beadxpress technology Ciência Rural Araújo,Ronyere Olegário de; Caetano,Alexandre Rodrigues. ABSTRACT: The increasing development of DNA sequencing and genotyping technologies has made possible to analyze the genomes of several species. Genomic studies of production animals have greatly increased the understanding of mechanisms that control the interactions of genetic and environmental factors involved in the expression of traits of economic importance. Several technologies have been presented by different companies for the genotyping of low-density SNP panels, which may be used in different applications with different goals, such as paternity testing, diagnosis of genetic diseases, and identification of genetically superior animals based on polymorphisms characterized in candidate genes. The present review critically analyzes the GoldenGate... Tipo: Info:eu-repo/semantics/article Palavras-chave: SNP genotyping; GoldenGate technology; Haplotypic blocks; Custom SNP panels; Association studies. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102005 Registros recuperados: 5 Primeira ... 1 ... Última

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